Abstract

REVEALING THE CHEMICAL BIOACTIVE COMPOUNDS OF MORINGA OLEIFERA BARK USING LC-MS ANALYSIS TO CONDUCT DETAILED BIOCHEMICAL ANALYSIS

Jyoti Rathore¹*, Kiran Thakur²

¹Department of Chemistry Govt E V Post Graduate College Korba(C.G),
²Department of Chemistry, Government Bilasa Girls PG College, Bilaspur (C.G)

ABSTRACT

Parts of Africa and Asia are home to the plant known as Moringa, commonly referred to as Moringa oleifera. Because of its therapeutic and nutritious qualities, it is also known as the "drumstick tree" or the "miracle tree." Bioactive substances like tannins, phenolic acids, flavonoids, and alkaloids are found in the bark. It is well recognized that these substances may have anti-inflammatory and antioxidant qualities. Liquid Chromatography-Mass Spectrometry (LC-MS) is a widely used technology for analyzing Moringa bark samples in order to identify and quantify different components. Combining the mass analysis capabilities of mass spectrometry with the separation capabilities of liquid chromatography yields a potent analytical technique known as LC-MS. While studies on the leaves, seeds, and roots of Moringa have been conducted, there is considerably little scientific data directly pertaining to the medical benefits of the plant's bark. As a result, it's crucial to use caution when using Moringa bark and to get the most recent information from trustworthy sources. Non polar extracts were prepared and analyzed for phytochemical in different extracts. M.oleifera extracts LC-MS chromatogram showed 105 peaks, which denote the presence of 105 phytochemical substances. Peak area, retention period, and molecular formula were used to identify the phytochemical substances. The main compounds identified are Quercetin, Kampeferol, Cinnamic acid , Beta sitosterol and Rhamnetin. 2,4-Dimethyldodecane, Eicosane, n-Hexadecanoic acid, Heneicosane, Octadecanoic acid, Tetracosane and many more presented in Chromatogram. Further the reported phytochemicals can be studied for pharmaceutical use after clinical trials and its finished product.

Keywords: Chromatogram, Phytochemical, Liquid Chromatography, Mass Spectrometry