Abstract

COMPREHENSIVE STUDY AND PREPARATION OF CULTURE AGAR MEDIA AND MICROSCOPIC EXAMINATION OF CULTURED LACTOBACILLUS BACTERIA BY USING STAINING TECHNIQUES

Sarthak Gupta, Akanksha Mishra*

Department of pharmacy, SAM Global University, Bhopal, MP India

ABSTRACT

Culture media is used in the laboratory for the cultivation of micro-organism supply for nutrients required for growth and maintenance The cultivation of a wide variety of bacteria is commonly done using nutrient agar, or NA, as a general-purpose medium. When studying bacteria, it's important to isolate them from different natural sources and facilitate their growth on artificial media in laboratories. The cultivation of bacteria means promoting the growth of the desired type of bacteria. A culture may be either pure or mixed. Based on this study it is concluded that agar medium is used for isolating (Lactobacillus) microbes and to determine characteristics of colonies. The appearance of colonies and changes that occur in the surrounding media help in the identification of bacterial species. For the identification of bacteria, different staining techniques are used along with microscopes in laboratories. In the differential staining process, There are several chemical stains utilized for better differentiation between different micro-organisms. Structures, cells, and sub-cellular structures. The gram stain is the differential stain for bacteria that is most widely employed (gram-positive and gram-negative). The amount and strain of useful bacteria in curd vary from place to place. The number of different lactobacillus bacteria isolated from curd preparation in India is high as 250 species. But can curd technically called probiotics and also can be consumed by a human with no harmful effects. Nowadays only lactic acid bacteria especially lactobacillus are used as probiotics. The intake of which can be beneficial for the immune system. Probiotics provide good bacteria to the gut, thus improving gut health. Therefore, This laboratory investigation looked at the lactobacillus bacteria's size, shape, and structure and it was compared to what differences were found between the cultured colonies of the bacteria and direct using a sample of fresh curd smear on the slide, where also lactobacillus is present. Gram-positive bacteria are generally considered to belong to the genus Lactobacillus.

Keywords: A Culture media (agar media), lactobacillus bacteria, micro-organism, staining techniques, microscopy, (gram positive- gram negative), probiotic, cultured colonies.