Abstract

GREEN SYNTHESIS AND CHARACTERIZATION OF SILVER NANOPARTICLE OF HYDROALCOHOLIC EXTRACT OF CUSCUTA REFLEXA

Alok Singh*, Adesh. A. Bawane, Brijyog, Manish Kumar Gupta

HCPG College, Varanasi, (U.P.)

ABSTRACT

The prevalence of infectious diseases has increased as a result of overuse and abuse of medications. AgNPs, along with other nanomaterials, have been studied in the defined post-antibiotic era to search for new agents that can help combat pathogenic microorganisms without promoting the appearance of new resistances. Additionally, green synthesis of AgNPs is preferred to offer a cost-effective, environmentally responsible, and cleaner synthesis method. Thus, this study deals with Green synthesis and characterization of silver nanoparticle of hydroalcoholic extract of Cuscuta reflexa. The AgNP combined with Cuscuta reflexa extract was synthesized & evaluated as per standard methods. Results showed that the entrapment efficiency for F3 Nanoparticle formulation was found to be 0.725±0.015. The Avg. particle size & Zeta potential of F3 was found to be 220.5 & - 38.5 mV respectively. The physical characteristics of F3 formulation was also touching idealistic parameters. From the three prepared formulations of gel the spreadibility & Viscosity for F2 was found to be 9.15±0.35 & 3025±8 respectively. Flavonoid content for F2 was found to be highest with value 0.728±0.032 mg/100mg while the pH of gel was noticed as 6.72±0.15. Results of in vitro drug release study also showed good results. The zero order & first order regression values of formulation F2 was found to be 0.953 & 0.914. The zone of inhibition at 100mg/ml of extract was found to be 12±0.47 while for same concentration the zone of inhibition for silver nanoparticle was found to be 15±0.75. This indicates that the cumulative antimicrobial effect of extract & AgNP is more appreciable.

Keywords: Cuscuta reflexa, AgNP, Antimicrobial, Green synthesis, C. albicans.