Abstract

ESTIMATION OF PHYTOCHEMICALS AND QUANTITATIVE STUDY PHENOLIC AND FLAVONOIDS CONTENT IN LEAVES EXTRACT OF WRIGHTIA TINCTORIA

Uttara Singh Bhandari*, Jaswinder Mehta, Ruchi Acharya, Bhawna Sharma, Peenu Mahendra Joshi

Department of Botany, Career College, Bhopal (M. P.)

ABSTRACT

Considering the vast reach of traditional medicine and a growing interest in self-produced medicines, the use of therapeutic plants is growing globally. One such plant Wrightia tinctoria R.Br. (Family: Apocynaceae), sometimes known as "Indrajau," is found all over the world and is particularly common in India. Traditionally it is used for many purposes. This study focuses on analysing it phytochemicals qualitatively & quantitatively. The leaves of plant were gathered & subjected to extraction by hydroalcoholic solvent. Qualitative & quantitative tests were then performed according to standard protocol. Results revealed that plant contain flavonoids, phenol, proteins, carbohydrates, saponins. The Rf value for quercetin standard was found to be 0.52. The bands obtained for long, short & normal light are 5,2 & 1 respectively. From the Rf value obtained from the extract it can be interpreted that extract contain range of flavonoid compounds along with quercetin. Total phenol & flavonoid content in Wrightia tinctoria extract was found to be 0.241 mg/100mg & 0.775 mg/100mg respectively. Thus, obtained results point towards fact that Wrightia tinctoria contain ample types & amounts of phytochemicals.

Keywords: Medicinal plants, Phytochemicals, Herbal medicines, Wrightia tinctoria, Phenol, flavonoids, TLC.