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Abstract

ANTIOXIDANT ACTIVITY AND HEPATOPROTECTIVE EFFECTS OF LUFFA ECHINATA ROXB. SEEDS EXTRACTS AGAINST ALCOHOL INDUCED HEPATIC DAMAGE

Vikash Rajak, Harshita Jain, Sunil K. Jain, Arpit Shrivastava*

Department of Pharmacology, Adina Institute of Pharmaceutical Sciences, Sagar (M.P.)-470001

ABSTRACT

The present study was done to determine the antioxidant activity and hepatoprotective effects of Luffa echinata (LE) seeds extracts/fractions against alcohol-induced hepatotoxicity in in vivo models. Total flavonoids content (TFC) and total phenolics contents (TPC) were determined using quercetin and tannic acid equivalents, as standard while antioxidant activities of different extracts/fractions against DPPH, hydroxyl (OH•) and inhibition of lipid peroxidation (LPO) were evaluated by using standard in vitro assays methods. In vitro active extract/fractions were subjected for the determination of hepatoprotective effects against alcohol induced liver damage. Among all extract/fractions, n-butanolic fraction (LEBF) showed marked possess potent antioxidant activity viz. DPPH (104.1g/ml), hydroxyl (OH•) (114.5 g/ml) and inhibition of lipid peroxidation (LPO) (148.6 g/ml), followed by methanolic extract (LEME) 158 g/ml, 184.7 g/ml and 207.4g/ml, respectively. Pre-treatment with LEBF significantly (p<0.001) restores the alcohol-induced altered serum liver enzymes activities as well as tissue antioxidant enzyme level, which is comparable to silymarin. Hepatoprotective potential was further supported by hepatic tissue histopathological results. Study results suggest that antioxidant activity and hepatoprotective effect of LEBF might be due to presence of polyphenols viz. TFC (49.68±0.72 mg QE/gm of extract) and TPC (112.17±0.72 mg GAE/gm of extract). In this study advocated that marked antioxidant and hepatoprotective activity of Luffa echinata seeds (i.e. LEBF) was exhibited due to the presence of polyphenols along with other compound.

Keywords: Alcohol; antioxidant; Luffa echinata; free radicals; polyphenols.


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