Login

Journal Front Page

News & Events

  • Call for article for January 2025 issue
    • Submissions of Review, Research Article, Short Communication and Case study are being accepted for the current and future issues before 15 December, 2024 of this journal by online process or editor@ajper.com, or ajper.editor@gmail.com. Further information can be found at www.ajper.com.
  • October 2024 Issue published
    • Dear Researcher, It Pleased to inform you that, Asian Journal of Pharmaceutical Education and Research, has successfully launch Volume 13, Issue 4 (October 2024 Issue). You can find the article on http://ajper.com/current_issue.php Article also invited for the Next coming Issue from your side. For More details Visit: www.ajper.com
  • Application For Reviewer
    • We are now going to reconstitute our editorial board members of Reviewer. Please send your resume at ajper.editor@gmail.com and be a part of the editorial board member as Reviewer.
  • AJPER Impact facor
    • It is to pleasure that Ajper SJIF imact facor has increse from 5.019 to 7.014. Also global impact factor incresed from 0.654 to 0.765.
  • ICV
    • AJPER Rank with Index Copernicus Value 61.10 due to high reputation at International Level

Abstract

METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF AMLODIPINE BESYLATE AND ATORVASTATIN CALCIUM BY UV SPECTROPHOTOMETRIC METHOD USING MIXED HYDROTROPIC PHENOMENA

Avanish Kumar*, Pushpendra Soni, Lavakesh K Omray

Radharaman Institute of Pharmaceutical Sciences, Bhopal (M.P)

ABSTRACT

A novel, simple, fast and reproducible UV spectrophotometric method was developed using 2M Sodium Benzoate: 2M Sodium acetate as hydrotropic solubilizing agent for the estimation of poorly water soluble drug Amlodipine besylate (AMD) and Atorvastatin calcium (ATV) in bulk and in pharmaceutical dosage form. After solubilising the Amlodipine besylate and Atorvastatin calcium in the selected hydrotropic agent, it was scanned in spectrum mode and Amlodipine besylate and Atorvastatin calcium showed linearity in the concentration range of 2-10 g/ml and 5-25g/ml at their respective maxima was selected as wavelength for estimation, considering the reproducibility and variability of the obtained result. The developed method was found to be linear in the range of 2-10 g/ml and 5 to 25μg/ml and with correlation coefficient (r2) of 0.999 and linear equation was Y=0.056X-0.002 and Y=0.002X-0.000 for Amlodipine besylate and Atorvastatin calcium. The mean percent label claims of synthetic mixture of Amlodipine besylate and Atorvastatin calcium in formulation estimated by the proposed method were found to be 99.046±0.284 to 99.828±0.308 and 98.18±0.386 to 98.40±0.706. These values are close to 100,indicating the accuracy of the proposed analytical method. Low values of standard deviation, percent coefficient of variation and standard error further validated the proposed method. Also the values of standard deviation, percent coefficient of variation and standard error were satisfactorily low. Result of precision at different level were found be within acceptable limits (RSD ‹ 2). Therefore, the methods can be used for routine monitoring of Amlodipine besylate and Atorvastatin calcium in industry in the assay of bulk drug and tablets.

Keywords: Amlodipine besylate and Atorvastatin calcium, Hydrotropy, Sodium acetate, Sodium Benzoate, Spectrophotometry.


[Full Text Article]