Abstract

Formulation development and Evaluation of Silvernanopaticle as effective antimicrobial agent

Yogita Patil*, Sunil Kumar shah, Prabhakar Budholiya, C. K. Tyagi

College of Pharmacy, Sri Satya Sai University of Technology & Medical Sciences, Sehore (M.P.)

ABSTRACT

Interest in green nanotechnology in nanoparticle biosynthesis is growing among researchers. Nanotechnologies due to their physicochemical and biological properties have applications in diverse fields, including drug delivery, sensors, optoelectronics and magnetic devices. Green synthesis of nanoparticles is an eco-friendly approach, which should be further explored for the potential of different plants to synthesize nanoparticles. The sizes of AgNPs are in the range of 1 to 100nm. Multi drug resistance has become an emerging and challenging issue for pharmaceutical industry as more and more bacteria are developing drug resistance towards many antibiotics, also over use of these synthetic drugs causes toxicity leading to further detrimental effects on human body. This study focused on the synthesis of silver nanoparticles, which is necessary for safe and effective exploitation of silver nanoparticles in collaboration with plant derived metabolites as a substitute for harmful synthetic drugs. Silver nanoparticles were synthesized using flowers of Curcuma angustifolia, synthesized silver nanoparticles were then subjected to check antibacterial activity against Propionibacterium acnes by well diffusion method. Phytochemical analysis of hydroalcoholic flowers of Curcuma angustifolia revealed the presence of phenol, flavonoids, proteins, carbohydrates, tannins and saponins. The total flavonoids and phenol content of hydroalcoholic extract Curcuma angustifolia was 0.876mg/100mg and 0.471mg/100mg/100mg respectively. Spectral techniques like UV-Vis, FT-IR, zetasizer, %EE and SEM were performed for the characterization of silver nanoparticles and results shows that AgNPs can be used as tool in combating the issue of drug resistance in future, as in comparison to broad-spectrum antibiotic, silver nanoparticles showed better bacteriostatic effect against bacteria.

Keywords: Green nanotechnology, Silver nanoparticles, Curcuma angustifolia, Phytochemical analysis, Well diffusion method.