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Abstract

Development and Validation of Ninhydrin Based Colorimetric Spectrophotometric Assay for Determination of Gentamicin in Pharmaceutical Formulation

Sri Prakash Mishra*1, Alok Pal Jain2

1School of Pharmacy and Research, Sitapur, (U.P.)

2Sarvepalli Radhakrishnan University, Bhopal (M.P)

ABSTRACT

A simple, accurate, cheap and quick spectrophotometric method was developed for the estimation of gentamicin in pharmaceutical pure and dosage forms. The method was based on the ninhydrin reaction with primary and secondary amines present in the gentamicin. This reaction produces a purple colour. The absorption of the gentamicin-ninhydrin mixtures at 324 nm had a linear relationship with the gentamicin concentration ranging from 10-100 μg/ml. The %RSD was less than 2%, showing high degree of precision of the proposed method. The methods were satisfactory applied for the determination of drugs in both bulk and pharmaceutical dosage forms. The colorimetric gentamicin assay reported herein is of great practical value because it is reproducible, sensitive, simple and extremely inexpensive.

Keywords: Gentamicin, Ninhydrin, Spectrophotometry, Pharmaceutical dosage forms


[Full Text Article]