Abstract

EVALUATION OF ANTI-INFLAMMATORY ACTIVITY OF METHANOLICLEAVES EXTRACT OF GARDENIA LATIFOLIA USING FORMALIN-INDUCED PAW EDEMA MODEL

Sonali Chourasiya*, Yashraj Yadav, Dr Sourabh Jain, Dr. Karunakar Shukla

College of Pharmacy, Dr. APJ University, Indore

ABSTRACT

Inflammation is a reaction of a living vascularized tissue to an injury. Conventional or synthetic drugs used in the treatment of inflammatory diseases are inadequate, it sometimes has serious side effects. So, number of herbal medicines is recommended for the treatment of inflammation that has no side effects. Hence our study focused to investigate the phytochemical analysis, quantification of bioactive compounds, in vitro free radical scavenging activity (DPPH radical method) and anti-inflammatory activity (formalin-induced paw edema) of methanolic leaves extract of Gardenia latifolia (G. latifolia) which has boundless medicinal properties. Qualitative analysis of various phytochemical constituents, quantitative analysis of total phenolics (Folins ciocalteau reagent method) and flavonoids (Aluminium chloride method), and in vitro free radical scavenging activity (DPPH radical method) were determined by the well-known test protocol available in the literature. For anti-inflammatory activity, wistar albino rats were used and divided into four groups of six animals each group and Group 1 was treated as manage (formalin (0.2 ml of 2% v/v freshly prepared formalin resolution in distilled water), group 2 was received diclofenac sodium 30mg/kg, p.o. group 3 were dealt with methanolic leaves extract of G. latifolia (MEGL) (100mg/kg, p.o.). group 4 were handled with methanolic leaves extract of G. latifolia (MEGL) (200mg/kg, p.o.). The thickness was measured earlier and after injecting the formalin everyday at a fixed time for seven consecutive days utilizing a vernier caliper. Phytochemical analysis revealed the presence of phenols, flavonoids, proteins and amino acids, carbohydrates, saponins, diterpines. The total phenolics content of methanolic leaves extract was (0.77mg/100mg), followed by flavonoids (0.60mg/100mg). The activities of methanolic leaves extract against DPPH assay method were concentration dependent with IC 50 values of ascorbic acid and extracts 35.44and 65.80μg/ml respectively. This plant also exhibits better anti-inflammatory activity. From the present observation, it is evidenced that G. latifolia would be an effective plant for the treatment of inflammatory reactions.

Keywords: Inflammation, Gardenia latifolia, Antioxidant, Formalin-induced paw edema, Phytochemical analysis