Abstract

PHYTOCHEMICAL SCREENING AND IN-VIVO ANTIDIABETIC ACTIVITY OF HYDROALCOHOLIC EXTRACT OF SEEDS OF LEPIDIUM SATIVUM LINN

Bhagwant Mewada*, Rajendra Chouksey, Harish Pandey, Prabhakar Budholiya

Sri Satya Sai University of Technology & Medical Sciences, Sehore (M.P.)

ABSTRACT

Diabetes mellitus (DM) is a global health problem and the incidence of DM is increasing at alarming rate all over the world. Many Indian medicinal plants have been reported to possess potential antidiabetic activity and could play important role in the management diabetes. This study sought to evaluate the antidiabetic potentials of hydroalcoholic extract of seed of L. sativum in diabetic rats. Qualitative analysis of various phytochemical constituents and quantitative analysis of total phenolics and flavonoids were determined by the well-known test protocol available in the literature. Quantitative analysis of phenolic and flavonoids was carried out by folins ciocalteau reagent and aluminium chloride method. Diabetes was induced in rats by Alloxan monohydrate (120 mg/kg; i.p.) administration. Oral treatment of hydroalcoholic extract of L. sativum using rat oral needle at 100 and 200mg/kg doses significantly (𝑃 < 0.001) decreased blood glucose and cholesterol levels in diabetic rats than control rats. In this study, Body weight, blood glucose, HDL, Total Protein, SGOT, SGPT, cholesterol and triglyceride were measured. The results showed that there was a significant decrease (P<0.01) in the blood glucose, cholesterol, total protein, SGOT, SGPT and triglyceride levels in diabetic rats when compared to the normal (control) rats and increase the level of HDL and body wt. In conclusion, the above actions might be responsible for the antidiabetic activity of extract due to presence of gallic acid and other biomarkers.

Keywords: Diabetes mellitus, Lepidium sativum, Phytochemical screening, Alloxan monohydrate