Abstract

EXTRACTION, PHYTOCHEMICAL SCREENING AND IN VIVO ANTIINFLLAMATRY ACTIVITY OF HYDROALCOHOLIC EXTRACT OF WHITE CATECHU

Sugam Pathak*, Harish Pandey, Prabhakar Budholiya

College of Pharmacy, Sri Satya Sai University of Technology and Medical Sciences, Sehore (M. P.)

ABSTRACT

Acacia catechu willdis an indigenous plant having tremendous medicinal properties and well-reviewed in Indian literature. Acacia catechu bark and heartwood has already been reported to possess manypharmacological activities such as astringent, antioxidant, treating fever, leucorrhoea, piles, erysipelas, haemoptysis, gonorrhea, hepatoprotective, antipyretic and chest infection ect.The antiinflammatory activity was evaluated by carrageenan-induced rat paw edema method. Acute toxicity of the extract (2000 mg/kg) was examined in wistar rats for 14 days. Qualitative analysis of various phytochemical constituents and quantitative analysis of total phenolics and flavonoids were determined by the well-known test protocol available in the literature. Quantitative analysis of phenolic and flavonoids was carried out by FolinsCiocalteau reagent and aluminium chloride method respectively. Phytochemical analysis revealed the presence of phenols, flavonoids, tannins, saponins, alkaloids. The total phenolics content of A. catechu extract was (1.984mg/100mg), followed by flavonoids (0.865mg/100mg) respectively. Hydroalcoholic extract up to 2000 mg/kg did not produce any toxic effects. The hydroalcoholic extract of A. catechu (100 and 200 mg/kg) inhibited the inflammation induced by carrageenan in rats in a dose dependent manner. The hydroalcoholic extract of A. catechu possesses a strong anti-inflammatory activity and may be considered an interesting source of effective anti-inflammatory compounds.

Keywords: Acacia catechu willd, Acute toxicity, Anti-inflammatory effect, Phytochemical screening, FolinsCiocalteau reagent.