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Abstract

DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF AMOXICILLIN TRIHYDRATE, METRONIDAZOLE AND FAMOTIDINE

Nidhi Jain Singhai*, Suman Ramteke

School of Pharmaceutical Sciences, Rajiv Gandhi Proudyogiki Vishwavidyalaya, Bhopal

ABSTRACT

The objective of the current study was to develop and validate a simple, accurate, precise and rapid reversed-phase HPLC method for simultaneous estimation of Amoxicillin trihydrate (AMOX), Metronidazole (METRO) and Famotidine (FAMO). The chromatographic separation of AMOX, METRO and FAMO were achieved on RP-HPLC having Luna C-18-ODS bonded column of length 250mm using UV detection at 267 nm. The optimized mobile phase consisted of a mixture of 0.03M disodium hydrogen phosphate buffer–acetonitrile (93:7, v/v) adjusted to pH 6.5 at a flow rate of 1.5 ml/min. The retention times were 2.560, 3.657 and 6.983 min for AMOX, FAMO and METRO respectively. The proposed method provided linear responses within the concentration ranges of 050µg/ml for Amoxicillin trihydrate & Metronidazole and 0-30µg/ml for Famotidine. The LOD values were 0.0252, 0.0098 and 0.0288 µg/ml and LOQ values were 0.0765, 0.0298 and 0.0875µg/ml for Amoxicillin trihydrate & Metronidazole and Famotidine, respectively. High recovery and low % coefficient of variation (COV) revealed the reliability of the method for quantitative study of the three drugs in combined dosage form.

Keywords: Amoxicillin trihydrate, Metronidazole, Famotidine, Simultaneous estimation, Reversedphase HPLC method.


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