Abstract

ANTI-DIABETIC ACTIVITY OF SILVER NANOPARTICLES SYNTHESIZED FROM ASHWAGANDHA ROOT AQUEOUS EXTRACT

Md. Imad Uddin*, Kavya G, Soundarya G, Ropa G, Sridhar CH

Department of pharmacology, Pulla Reddy Institute of Pharmacy, Domadugu, SangaReddy, Telangana, India-502313.

ABSTRACT

According to traditional medicine, Withania somnifera also known as Indian ginseng is a plant with a cure for many diseases. This study was conducted to develop an ecofriendly, cheap and effective procedure for the green synthesis of silver nanoparticles (AgNPs) form ashwagandha root aqueous extract (ARAE) and evaluating their in-vitro anti diabetic activity. Addition of ARAE to AgNO3 (1mM) solution kept on stirring at 60oC resulted in the formation of Ashwagandha Root Aqueous Extract Nano particles (ARAENP). Synthesized nanoparticles are subjected to characterization by various procedures. UV-Visible spectroscopy showed absorption peak at 440nm which was absent in ARAE and AgNO3. FTIR spectroscopy revealed the absorption peaks of different functional groups involved in the formation of ARAENP and further emphasizes role of withanolides in formation of ARAENP. Scanning Electron Microscopy (SEM) revealed average size of ARAENP as 123.23nm. 2 ߠpeaks at 28.98o, 39.80o, 42.24o, 64.42o and 79.76o were identified by X-ray Diffraction studies (XRD). Stability of ARAENP was determined by zeta potential which was found to be -10mV. Successfully characterized ARAENP were evaluated for their in-vitro anti diabetic potential by α-amylase and α –glucosidase inhibitory activities. Acarbose was used as a standard. Inhibitory potential of ARAE and ARAENP against α-amylase and α –glucosidase proves their therapeutic role. Inhibition of enzyme was probably due to the withanolides capped on ARAENP.

Keywords: ?-Amylase, ? –Glucosidase, Ashwagandha, silver nanoparticles, Diabetes mellitus.