Abstract

EXTRACTION, PHYTOCHEMICAL INVESTIGATION AND WOUND HEALING ACTIVITY OF THE HYDROALCOHOLIC FLOWER EXTRACTS OF CALENDULA OFFICINALIS

Md. Osama Belal, Deepak Kumar Basedia, B. K. Dubey

Technocrats Institute of Technology, Pharmacy, Bhopal (M.P.)

ABSTRACT

This study focuses on the extraction, phytochemical investigation, and wound healing activity of the hydroalcoholic flower extracts of Calendula officinalis. Calendula officinalis, commonly known as marigold, is a medicinal plant with reputed wound healing properties. The hydroalcoholic extracts of its flowers were obtained using a suitable extraction method. The extracts were subjected to phytochemical screening to identify the presence of various secondary metabolites such as flavonoids, terpenoids, saponins, tannins, and alkaloids. The results of the phytochemical screening revealed the presence of flavonoids, terpenoids, saponins, and tannins in the hydroalcoholic flower extracts of Calendula officinalis. These compounds have been previously reported to possess wound healing properties. The wound healing activity assessment demonstrated significant improvements in wound closure, increased epithelialization, and enhanced tensile strength in the animals treated with the extracts compared to the control group. Histopathological examination revealed accelerated tissue regeneration and increased collagen deposition in the extract-treated group. Based on these findings, it can be concluded that the hydroalcoholic flower extracts of Calendula officinalis possesses potent wound healing activity. The presence of bioactive compounds, such as flavonoids and terpenoids, may contribute to the observed effects. Further investigation is warranted to isolate and identify the specific active components responsible for the wound healing activity of Calendula officinalis extracts.

Keywords: Calendula officinalis, Wound healing activity, Hydroalcoholic, Flower Extract