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Abstract
DEVELOPMENT AND VALIDATION OF NOVEL METHOD FOR ESTIMATION OF HERBAL DRUG- CAJANIN
Shivani kashid*, Neelam Jain
Department of Pharmaceutics, Faculty of Pharmacy, Oriental University, Indore-453555, Madhya Pradesh, India
ABSTRACT
Many Natural drugs have been extensively used recently for the treatment of different diseases and health problems including cancers. The use of natural drug has been well written in the history of Indian medicine however, extraction of the medicinal drug from the crude plant parts are difficult. Therefore, there is a requirement for the development of a method for extraction and evaluation of plant-based drugs. Our research is based on the development of a method for extraction of anticancer drug from Cajanus cajan and validation of the developed method. The plant was obtained from the local market and dried under shade for 15 days. After proper drying extraction was performed by the process of maceration with methanol as a solvent for 72 hours. Different physicochemical and phytochemical parameters were evaluated spectrometrically using HPLC. The developed method of extraction and evaluation was validated for linearity, precision, accuracy, specificity, robustness, ruggedness, and a few others. Results drawn from the study indicated the amount of the sample extracted to be 24.69mg and the percentage purity was found to be 98.76%. Linearity was determined in the range of 5 - 25 μg/ml. The correlation coefficient value was >0.99. The developed method was found to be accurate and precise from the % RSD values determined. The LOD and LOQ value for cajanin was found to be 0.044 µg/ml, and 0.134 µg/ml, respectively. This resembles that the proposed method was sensitive.
Keywords: Anticancer, Apoptosis, Validation, LOD, Cajanin, Extraction
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