Abstract

A PRELIMINARY SCREENING OF BACTERIAL ISOLATES PRODUCING AN ONCOTHERAPEUTIC ENZYME ASPARAGINASE FROM SOME SEWAGE WATER SAMPLES OF BHOPAL, M.P. INDIA

Shreya Tiwari¹, Rakesh Mehta¹, Ayush Kushwaha² and Rajnandani Katariya²*

¹Department of Biotechnology, Govt. MGM P.G. College, Itarsi, M.P India
² Biomedical & Biopharmaceutical Division, Lenience Biotech Lab, Bhopal, M.P. India)

ABSTRACT

The production of enzymes is a pursuit central to the modern biotechnology industry. Asparaginase is one such important enzyme finds their use in the pharmaceutical, biosensor and food industries and has anticarcinogenic potential for the treatment of acute lymphoblastic leukemia, lymphomas and other cancers. Asparaginase are naturally occurring enzymes expressed and produced by animal tissues, bacteria, plants, and in the serum of certain rodents, but not in mankind. Erwinia carotovora, Pseudomonas stutzeri, Pseudomonas aeruginosa, E. coli etc has been known to produce L-asparaginase, though its commercial production is restricted to use of Erwinia chrysanthemi and E. coli as per the latest information. Thus the present work was intended to screen out certain new bacterial isolates with in vitro asperginase producing potential from sewage water sources. The sewage water samples were collected from 5 different location of Bhopal and were subjected to brain heart infusion media containing amino acid asparagine and phenol red indicator. The bacterial isolates forming pink or red colonies or hallow around indicating asparaginase producing capability were picked and pure cultured. The three asparaginase positive isolates one from M.P. Nagar & other two from AIIMS sewage sample reported to be bacillus and coccus respectively on Gram’s staining which when subjected to biochemical characterization using catalase test, IMVC test and carbohydrate fermentation tests does not matches with the biochemical characteristics of standard E.coli and S. aureus. The outcomes of this preliminary screening encourages to further investigate the sewage water samples to screen out the possibly new & efficient asparaginase producing isolates with complete biochemical & molecular characterization in view of commercial prospects.

Keywords: L-asparaginase, anticarcinogenic, enzyme production, wastewater