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Abstract

ESTIMATION OF BIOACTIVE COMPOUND USING RP-HPLC AND ANTIBACTERIAL ACTIVITY OF HYDROALCOHOLIC EXTRACT OF SWERTIA CHIRAYITA (ROXB. EX FLEMING)

Ankita Shrivastava1, Rashmi Mishra1, Malika Pal1, Prabhat Jain2

ABSTRACT

Utilization of herbs for medicinal purpose started in the early history of mankind several thousand years ago. The worldwide increase of multidrug resistance has impaired the current antimicrobial therapy and warranting the search for other alternatives. Swertia chirayita is one of the potential medicinal plants of the family Gentianaceae in traditional medicine. Due to its high demand and scarcity, trade of chirayita is affected by adulterants. The present study was undertaken to find the antibacterial activity, phytochemical presence and quercetin was detected in hydroalcoholic leave extract of Swertia Chirayita under study by using RP-HPLC analysis. Hydroalcoholic leave extract of Swertia Chirayita were evaluated for antibacterial activity against Two-gram negative strain like Salmonella bongori, Proteus Vulgaris and one-gram positive strain like E. Faecalis using agar diffusion method (well method). The significant antibacterial activity of the active extract was compared with standard antibiotic ciprofloxacin (10-30 μg/ml). The antibacterial activity was determined by measuring the diameter of the zone of inhibition in term of millimeter (mm). The preliminary phytochemical studies and quantitative analysis of flavonids were performed by well reported method. The phytochemical analysis showed the presence of tannins, glycosides, flavonoids and alkaloids ect. It is concluded that the antibacterial activity showed by the plant is due to the presence of these phytochemicals. For future studies, phytochemcials responsible for these activities can be isolated and modified for pharmacological purpose.

Keywords: Swertia Chirayita, Quercetin, RP-HPLC, Antibacterial Activity, Flavonoids.


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