Abstract

HEPATOPROTECTIVE AND ANTIOXIDANT ACTIVITY OF CNIDOSCOLUS CHAYAMANSA AGAINST D-GALACTOSAMINE INDUCED LIVER DAMAGE IN RATS

Supyar Singh, Salaj Khare, B. K. Dubey, Amit Joshi, Amit Jain, Grijesh Pandey

Technocrats Institute of Technology-Pharmacy Education and Research, Bhopal (M.P.)

ABSTRACT

In the deficiency of dependable liver-protective drugs in contemporary medicine, a large number of medicinal preparations are recommended for treatment of liver disorders. Cnidoscolus chayamansa Mc Vaugh (Euphorbiaceae) is a medicinal and edible plant known as Chaya, is commonly used as an anti-inflammatory, antiprotozoal, antibacterial agent and as a remedy for respiratory illness, gastrointestinal disorders and vaginal infections related with the inflammation process. Although C. chayamansa is one of the most used and valued medicinal plants, only a few studies on documenting its pharmacological properties can be found. The antioxidant and hepatoprotective activities of ethanolic extracts of C. chayamansa leaves are evaluated here. Ethanolic extract of C. chayamansa (EECP, 200and400mg/kg,p.o.) was evaluated for its hepatoprotective and antioxidant activity in d-galactosamine (d-GalN)-induced hepatotoxicity in rats. Biochemical and histopathological studies were performed to assess hepatoprotective activity. d-GalN administration induced hepatotoxicity in rats which was manifested by increased levels of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), total bilirubin (TB), Gamma-glutamyl transpeptidase(GGTP) and decrease levels of Total protein(TP) and Total albumin(TA).In liver homogenate, there was significant decrease in SOD, CAT and GPx levels and increase in LPO levels were observed in animals treated with galactosamine as compared to normal control group. Pretreatment with EECP significantly protected the liver in d-GalN administered rats. EECP significantly elevated antioxidant enzymes like superoxide dismutase, catalase, glutathione peroxidase and decreased lipid peroxidation levels in liver. Histological studies showed that EECP at 400 mg/kg attenuated the hepatocellular necrosis in d-GalN intoxicated rats. From this study, it can be concluded that the ethanolic extract of C. chayamansa is not only an effective hepatoprotective agent, but also possesses significant antioxidant activity.

Keywords: Cnidoscolus chayamansa, Hepatoprotective, Antioxidant activity, D-galactosamine, Biochemical and histopathological studies