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Abstract

AJPER Oct – Dec. 2018, Vol 7, Issue 4 (94-106) RP-HPLC AND UV SPECTROPHOTOMETRIC METHODS FOR ESTIMATION OF TICAGRELOR IN PHARMACEUTICAL FORMULATIONS

Harpal Narware*, Kapil Malviya, Brijesh Sirohi, Lavakesh Kumar Omray

Radharaman Institute of Pharmaceutical Sciences, Bhopal

ABSTRACT

An accurate, precise, sensitive and reproducible High-performance liquid chromatographic and UV spectrophotometric method were developed and validated for the quantitative determination of ticagrelor, a novel antiplatelet agent used in acute coronary syndrome in bulk drug and pharmaceutical formulation. Chromatography was carried out by isocratic technique on a reversed-phase Thermo C18 (250 × 4.6 mm, 5μm) column with mobile phase consisting of 20 mM KH2PO4: acetonitrile (pH 3.0 with OPA) in the ratio of 20:80 v/v at flow rate of 1.0 ml/min. The retention time for ticagrelor was 8.102± 0.3 min. The UV spectrophotometric determinations were performed at 282 nm using water as a solvent. The proposed methods were validated according to International Conference on Harmonization ICH Q2 (R1) guidelines. The linearity range for ticagrelor was 5-25 μg/ml for HPLC and UV method. The linearity of the calibration curves for each analyte in the desired concentration range was good (r2 >0.999) by both the HPLC and UV methods. Both the methods were accurate and precise with recoveries in the range of 98 and 99 % and relative standard deviation <2 %. The developed methods were successfully applied for determination of ticagrelor in tablets.

Keywords: Pharmaceutical formulation, Ticagrelor, RP-HPLC, UV


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