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  • October 2018 issue published
  • Dear Researcher, It Pleased to inform you that, Asian Journal of Pharmaceutical Education and Research, has successfully launch Volume 7, Issue 4 (October Issue). You can find the article on http://ajper.com/current_issue.php Article also invited for the Next coming Issue from your side. For More details Visit: www.ajper.com
  • Call for Article for January Issue
  • Submissions of Review, Research Article, Short Communication and Case study are being accepted for the current and future issues before 25 December, 2018 of this journal by online process or editor@ajper.com, or ajper.editor@gmail.com. Further information can be found at www.ajper.com.
  • AJPER Impact facor
  • It is to pleasure that Ajper SJIF imact facor has increse from 4.101 to 5.019. Also global impact factor incresed from 0.654 to 0.765.
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  • AJPER Rank with Index Copernicus Value 61.10 due to high reputation at International Level

Abstract

AJPER Oct Dec. 2018, Vol 7, Issue 4 (94-106) RP-HPLC AND UV SPECTROPHOTOMETRIC METHODS FOR ESTIMATION OF TICAGRELOR IN PHARMACEUTICAL FORMULATIONS

Harpal Narware*, Kapil Malviya, Brijesh Sirohi, Lavakesh Kumar Omray

Radharaman Institute of Pharmaceutical Sciences, Bhopal

ABSTRACT

An accurate, precise, sensitive and reproducible High-performance liquid chromatographic and UV spectrophotometric method were developed and validated for the quantitative determination of ticagrelor, a novel antiplatelet agent used in acute coronary syndrome in bulk drug and pharmaceutical formulation. Chromatography was carried out by isocratic technique on a reversed-phase Thermo C18 (250 × 4.6 mm, 5μm) column with mobile phase consisting of 20 mM KH2PO4: acetonitrile (pH 3.0 with OPA) in the ratio of 20:80 v/v at flow rate of 1.0 ml/min. The retention time for ticagrelor was 8.102± 0.3 min. The UV spectrophotometric determinations were performed at 282 nm using water as a solvent. The proposed methods were validated according to International Conference on Harmonization ICH Q2 (R1) guidelines. The linearity range for ticagrelor was 5-25 μg/ml for HPLC and UV method. The linearity of the calibration curves for each analyte in the desired concentration range was good (r2 >0.999) by both the HPLC and UV methods. Both the methods were accurate and precise with recoveries in the range of 98 and 99 % and relative standard deviation <2 %. The developed methods were successfully applied for determination of ticagrelor in tablets.

Keywords: Pharmaceutical formulation, Ticagrelor, RP-HPLC, UV


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