Login

Journal Front Page

News & Events

  • April 2024 Issue delayed
    • Dear Researcher, It Pleased to inform you that, Asian Journal of Pharmaceutical Education and Research, Volume 13, Issue 2 (April 2024) Issue) schedule published on 15 April 2024 is delay due to some technical problem. It will published on 22 April 2024. Sorry for inconvenience. For other details You can vist the website http://www.ajper.com
  • Call for article for April 2024 issue
    • Submissions of Review, Research Article, Short Communication and Case study are being accepted for the current and future issues before 15 March, 2024 of this journal by online process or editor@ajper.com, or ajper.editor@gmail.com. Further information can be found at www.ajper.com.
  • January 2024 Issue published
    • Dear Researcher, It Pleased to inform you that, Asian Journal of Pharmaceutical Education and Research, has successfully launch Volume 13, Issue 1 (January 2024) Issue). You can find the article on http://ajper.com/current_issue.php Article also invited for the Next coming Issue from your side. For More details Visit: www.ajper.com
  • Application For Reviewer
    • We are now going to reconstitute our editorial board members of Reviewer. Please send your resume at ajper.editor@gmail.com and be a part of the editorial board member as Reviewer.
  • AJPER Impact facor
    • It is to pleasure that Ajper SJIF imact facor has increse from 5.019 to 7.014. Also global impact factor incresed from 0.654 to 0.765.
  • ICV
    • AJPER Rank with Index Copernicus Value 61.10 due to high reputation at International Level

Abstract

DEVELOPMENT AND VALIDATION OF A STABILITY INDICATING RP-HPLC ASSAY METHOD FOR DETERMINATION OF LAMOTRIGINE IN TABLET FORMULATION

Rashid Ahmad *, Pushpendra Soni, Lavakesh Kumar Omray

Radharaman Institute of Pharmaceutical Sciences, Bhopal

ABSTRACT

The objective of the current study was to developed simple, precise and accurate isocratic reversed-phase stability indicating high performance liquid chromatography (HPLC) assay method and validated for determination of lamotrigine in solid pharmaceutical dosage forms. Isocratic RP-HPLC separation was achieved on a Thermo C18 column (250 mm x 4.6 mm i.d., 5 μm particle size) using mobile phase of Acetonitrile: Buffer (1.75 gm KH2PO4 in 1000 ml of water add 1 ml of TEA and adjust the pH 6 with OPA, 40:60, v/v) at a flow rate of 1.0 ml/min and the detection was carried out at 225 nm by using photo-diode array detector. The drug was subjected to oxidation, hydrolysis, photolysis and heat to apply stress condition. The method was validated for specificity, linearity, precision, accuracy, robustness and solution stability. The retention time was 5.013 + 0.3 min and the method was linear in the drug concentration range of 5-25 μg/ml with a correlation coefficient 0.999. The percentage relative standard deviation in accuracy and precision studies was found to be less than 2%. The accuracy (recovery) was between 99.830and 100.208 %. Degradation products produced as a result of stress studies did not interfere with detection of lamotrigine and the assay can thus be considered stability indicating.

Keywords: Lamotrigine, Method Development, Validation, RP-HPLC, Stability indicating


[Full Text Article]