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Abstract

VALIDATED RP HPLC METHOD DEVELOPMENT FOR THE ESTIMATION OF LOSARTAN POTASSIUM IN MARKETED FORMULATION

Amit Kumar Jain*1 B.K. Dubey1, Amit Joshi1, Salaj Khare1, Rajesh Bhardwaj1, Prabhat Jain2

1Technocrats Institute of Technology Pharmacy Education and Research, Bhopal (M.P.), India.
2Scan Research Laboratory, Indrapuri, Bhopal, India.

ABSTRACT

A simple, reliable, rapid, precise, sensitive and validated RP-HPLC method has been developed to determine Losartan in pharmaceutical dosage form. Chromatographic separation achieved isocratically Thermo C₁₈ column (4.6 x 250mm, 5μ particle size) as the stationary phase with a flow rate of 1.0 ml/min and using a UV detector to monitor the eluate at 286 nm. The mobile phase consisted of Methanol: Acetonitrile (10:90v/v) enabled separation of the drug. Parameters such as linearity, precision, accuracy, recovery, specificity and robustness are studied as reported in the ICH guidelines. The retention times for Losartan was found to be 3.305±0.5 min. Linearity for Losartan was in the range of 5-25μg/ml. The mean recoveries obtained for Losartan was 100.22% and RSD was less than 2. The correlation coefficients for all components are close to 1. Developed method was found to be accurate, precise, selective and rapid for estimation of Losartan in pharmaceutical dosage form.

Keywords: Losartan, RP-HPLC, Validation.


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