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ESTIMATION OF BIO-ACTIVE CONSTITUENTS BY RP-HPLC
Chaitanya Sarathe *1, Suparna Ghosh1 Shweta Sharma1, Prabhat Jain2
Career College, Bhopal (M.P.) Pin- 462023, India
Qualitative phytochemical analysis was done ariel part of plant of Eclipta alba. The test confirms the presence of various phytochemicals like Alkaloids, Glycoside, flavonoids, saponins, and terpenoids in hydrolcoholic extract. Quantitative analysis of bioactive constituent rutin also estimated by RP-HPLC method. The results suggest that the hydroalcoholic extract shows the presence of rutin during screening. The developed method for the estimation of rutin method has the following advantages: rapid extraction, easy sample preparation and short time injections.
Keywords: Qualitative, Quantitative, Bioactive, Eclipta alba.
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