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EVALUATION OF ACUTE, SUBACUTE AND SUBCHRONIC TOXICITY OF POLYGONUM GLABRUM
Raja. Sundararajan*, Ramya. I
Department of pharmaceutical chemistry, GITAM institute of pharmacy, visakhapatnam, Andhra pradesh, India.
The aim of present study was to evaluate the safety dose range of methanol extract of Polygonum glabrum by acute, subacute and subchronic oral toxicity studies on animal model. In acute toxicity study, the animals were administered with an oral dose (2000 mg/kg) of methanol extract of Polygonum glabrum daily for a period of 15 days. In subacute and subchronic toxicity studies three doses of methanol extract of Polygonum glabrum (250, 500, and 1000 mg/kg) was administered to animals for 28 days and 90 days orally. Behavioral changes, adverse effects and mortality were recorded during the period of investigation. Body weight, organ weight, hematological, biochemical and histopathological studies were performed and compared with control group. In acute toxicity study, the animals did not show any changes in general behavior or any signs of toxicity. Also, no mortality was recorded. Methanol extract of Polygonum glabrum did not cause any significant difference (p > 0.05) in the body weight, relative organ weight, hematological, biochemical parameters and gross abnormalities during the 28 days study. However, in the 90-day study a significant increase in white blood cell and platelets were observed. Biochemical parameters such as total protein showed significant increase while creatinine and uric acid levels showed significant decrease. The results suggest that methanol extract of Polygonum glabrum is not likely to produce any significant toxic effect in animal models on oral administration.
Keywords: Polygonum glabrum, Acute, Subacute, Subchronic toxicity, Biochemical analysis, Hematological parameters.
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