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PHYTOCHEMICAL SCREENING, TOTAL PHENOLIC AND TOTAL FLAVONOID CONTENT AND ANTIOXIDANT ACTIVITY OF LAVANDULA STOECHAS LINN
Brijesh Sirohi* Rakesh Sagar
Vedica College of Pharmacy, R.K.D.F. University, Bhopal (M.P.)
Lavandula Stoechas is a Plant of the Lamiaceae/Labiatae family. The aim of this study was to screen the Phytochemicals, to evaluate the total flavonoid and total Phenolic contents as well as antioxidant activity of hydroalcoholic extract of Lavandula Stoechas. Plant material was extracted by using hydroalcoholic solvent for 2 h and repeated 3 times. Total flavonoid content was determined by aluminium chloride colorimetric assay on 420 nm. Total Phenolic content was determined with Folin-Ciocalteu 1:4 on 765 nm using microplate reader. Antioxidant activity was determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenger methods.Phytochemical screening showed that all of samples positively contain alkaloid, glycoside, flavonoid, phenolics, carbohydrate, diterpenes and saponin. Total flavonoid content was found to be 9.31(QEmg/100mg) whereas total Phenols was found to be 5.52 (GAEmg/100mg). The hydroalcoholic extract displayed DPPH free radical scavenging activity with76.41±0.47IC50 value, which could be related to its higher Phenolic content. It can be hypothesised that the high contents of Phenolic compounds of Lavandula Stoechas indicated that these compounds contribute to the antioxidant activity and can be regarded as promising plant species for natural sources of radical scavenging activity with potential value for treatment of many life threatening diseases.
Keywords: Lavandula Stoechas Linn, Phytochemical Screening, DPPH, Total Flavonoid, Total Phenolic content.
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