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D. Gupta*, C. Kushwah, A. Joshi, K. Malviya, S. Malviya, A. Kharia

Modern institute of Pharmaceutical Sciences, Alwasa, Behind Rewti Range, Sanwer Road, Dist. Indore (MP) Pin 453111


The study deals with the evaluation of anti-anemic activity of oral administration of hydro-alcoholic extract of fruit of Brassica oleracea at different dosage levels (250 & 500mg/kg body weight) on female Wistar rats using various paradigms of anemia. India is one of the richest sources of herbal plants and seeds. Many of them were used as traditional and folk medicine and as a natural remedy to cure various health problems in early days. In the present study, the extraction of fruit of Brassica oleracea was carried out with Soxhlet apparatus. The phytochemical screening showed the presence of phytoconstituents like triterpenoids, flavonoids, alkaloids, carbohydrate, protein-amino acid, saponins, mucilages and fixed oils in hydro-alcoholic of fruit of Brassica oleracea (L). The anti-anemic activity in rats was done by using the chemical phenylhydrazine. Phenylhydrazine was given to the experimental group (24 rats) as oral administration of 100mg /kg daily for a period of eight days to induce anemia and the remaining rats (6 rats) serves as normal control. Phenylhydrazine treated rats with Haemoglobin concentration of <14g/dl were considered as anemic and are included in the study. The hydro-alcoholic extract of fruit of Brassica oleracea was given to two experimental groups (IV and V group) at two different dosage levels of (250 mg/kg body weight and 500 mg/kg body weight) for a period of one month. The effect of hydro-alcoholic extract of fruit of Brassica oleracea in Wistar rats has shown significant increase in the level of hematological parameters after the period of treatment.

Keywords: Hydro-alcoholic extract, Brassica oleracea, Phytochemical screening, Anemia, Anti-anemic activity

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