PHYTOCHEMICAL SCREENING AND THIN-LAYER CHROMATOGRAPHIC STUDIES OF GLORIOSA SUPERBA LINN. SEED EXTRACT
ShailjaKamle*, Manjul Verma, Mahima Chaudhary, Nazi Qidwai
Department of Chemistry, Sarojini Naidu Government Girls P.G. (Autonomous) College, Bhopal (M.P.)
Gloriosa superba seed Linn. is one of the important medicinal plants now in endangered list. This plant is widely used for several ethano-medicinal purposes by tribal peoples and traditional practitioners. The present study deals with the phytochemical screening and thin-layer chromatographic studies of Gloriosa superba seed extract belonging to family Colchicaceae. Phytochemical screening determination by some chemical tests and thin layer chromatographic study was carried out by using various solvent system of varying polarity. Phytochemical screening reflects the presence of alkaloids, flavonoids, diterpenes and proteins in hydroalcoholic extract. Thin layer chromatographic studies of the Gloriosa superba seed extracts constituted different colored phytochemical compounds with different Rf values. The result obtained in present study indicated Gloriosa superba seed as a rich source of natural antioxidants and provides evidence that solvent extract of Gloriosa superba seed contains medicinally important bioactive compounds and this justifies the use of plant species as traditional medicine for treatment of various diseases.
Keywords: Gloriosa superba, Seed extract, Phytochemical, TLC.
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