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PHYTOCHEMICAL SCREENING AND THIN LAYER CHROMATOGRAPHIC STUDIES OF ANNONA SQUAMOSA (SEEDS), AZADIRACHTA INDICA (LEAVES) AND LAVANDULA ANGUSTIFOLIA (FLOWER) AQUEOUS EXTRACT
Purva Tiwari*, Amit Joshi, B. K. Dubey
T.I.T. College of Pharmacy, Bhopal (M.P.)
India being a rich and varied flora of medicinal plants since the Vedic period. The present study deals with the phytochemical screening and thin layer chromatographic studies of Annona squamosa, Azadirachta indica and Lavandula angustifolia aqueous extract belonging to family Annonaceae, Meliaceae and Lamiaceae respectively. Phytochemical screening determination by some chemical tests and thin layer chromatographic study was carried out by using various solvent system of varying polarity of hexane, chloroform, ethyl acetate, acetone and methanol extracts. Phytochemical screening reflects presence of alkaloids, glycosides, saponins, phenolic compounds, tannins, phytosterols, carbohydrates, proteins, amino acids, flavanoids, quinones and terpenoids shows different types of results in different plant extracts. Thin layer chromatographic studies of the Annona squamosa, Azadirachta indica and Lavandula angustifolia aqueous extracts constituted different colored phytochemical compounds with different Rf values. The aqueous extracts in the drug is carried out to establish the biomarker compound. The result obtained in present study indicated Annona squamosa, Azadirachta indica and Lavandula angustifolia as a rich source of natural antioxidants and provides evidence that aqueous extract of Annona squamosa, Azadirachta indica and Lavandula angustifolia contains medicinally important bioactive compounds and this justifies the use of plant species as traditional medicine for treatment of various diseases.
Keywords: Annona squamosa, Azadirachta indica, Lavandula angustifolia,, Phytochemical, Retention factor, TLC Studies. Phytochemical, Chromatography.
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