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Mamta Maheshwari1, Ketki Verma1, Kavita Soni1, Prabhat Jain*2

1. Department of Chemistry, Sarojini Naidu Government Girls P.G. (Autonomous) College, Bhopal (M.P.)
2. Scan Research Laboratories, Bhopal (M.P.)


An attempt has been made to develop a simple, sensitive and rapid spectrophotometric method of analysis for lomefloxacin in pharmaceutical dosage form using different media such as 0.1N NaOH, 0.1N HCl buffer as solvent system. These solvent systems were used to dissolve lomefloxacin and 0.100 mg/mL stock solutions were prepared for each solvent system. Lomefloxacin solution was scanned with UV spectrophotometer and the absorption maximum (λmax) was found to be 287 nm. These solvent systems could be used for routine analysis of lomefloxacin in both research laboratories and pharmaceutical industries. The methods were validated statistically as per the ICH guidelines which yielded good results concerning range, precision, accuracy, specificity and repeatability. The proposed method has been applied successfully for the determination of lomefloxacin in pharmaceutical dosage forms. No significant interference was observed from excipients, coloring and flavouring agents commonly used in the formulation. It was, thus, concluded that the proposed method is new, simple, cost effective, accurately, precise, safe and free from pollution and can be successfully employed in the routine analysis of lomefloxacin in bulk drug and tablet dosage forms.

Keywords: Lomefloxacin, spectrophotometry, analysis, reproducible.

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