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Abstract

DETERMINATION OF TOTAL PHENOLIC, FLAVONOID CONTENT, ANTIOXIDANT AND ANTIMICROBIAL ACTIVITY OF GLORIOSA SUPERBA SEED EXTRACT

Arpana Gaur Mishra*1, Richa Singh1, Neha Patil1, Geeta Parkhe2

1. Department of Chemistry, Sarojini Naidu Government Girls P.G. (Autonomous) College, Bhopal (M.P.)
2. Scan Research Laboratories, Bhopal (M.P.)

ABSTRACT

Plants are a rich source of phenolic compounds and one of the most important nature-based antioxidants. The compounds derived from plant-based extracts are an important pharmaceutical resource. This study was conducted to investigate the antioxidant activity and total phenolic and flavonoid content and to investigate the antibacterial effects of Gloriosa superba seed extract. In this study, Gloriosa superba was gathered and extracted by Soxhlet extraction using hydroalcoholic solvent. Total phenol content was measured by Folin-Ciocalcteu reagent and gallic acid standard, total flavonoid content was measured. Antimicrobial effects were investigated by disk diffusion with reference to ciprofloxacin. In this study, investigating the effects of different concentrations of hydroalcoholic Gloriosa superba extract on pathogenic bacteria demonstrated that this extract exerted great inhibitory effects on both bacteria. The results indicated that the plants tested may be potential sources for isolation of natural antioxidant and antimicrobial compounds.

Keywords: Antioxidant activity, Total phenolic content, Flavonoid content, Antimicrobial effect, Gloriosa superba linn.


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