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Abstract

DETERMINATION OF TOTAL PHENOLIC, FLAVONOID CONTENT AND ANTIMICROBIAL ACTIVITY

Alka Pradhan*, Kavita Mehra, Deepa Khandale, Jyoti Warkhade

Department of Chemistry, Sarojini Naidu Government Girls P.G. (Autonomous) College, Bhopal (M.P.)

ABSTRACT

Plants are a rich source of phenolic compounds and one of the most important nature-based antioxidants. The compounds derived from plant-based extracts are an important pharmaceutical resource. This study was conducted to investigate the antioxidant activity and total phenolic and flavonoid content and to investigate the antibacterial effects of Litsea glutinosa bark extract. In this study, Litsea glutinosa was gathered and extracted by Soxhlet extraction using hydroalcoholic solvent. Total phenol content was measured by Folin-Ciocalcteu reagent and gallic acid standard, total flavonoid content was measured and antioxidant activity was investigated by DPPH with reference to ascorbic acid. Antimicrobial effects were investigated by disk diffusion with reference to ciprofloxacin. In this study, investigating the effects of different concentrations of hydroalcoholic Litsea glutinosa extract on pathogenic bacteria demonstrated that this extract exerted great inhibitory effects on both bacteria. The results indicated that the plants tested may be potential sources for isolation of natural antioxidant and antimicrobial compounds.

Keywords: Antioxidant activity, Total phenolic content, Flavonoid content, Antimicrobial effect, Litsea glutinosa (Lour).


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